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mutant tcf lef reporter plasmid  (Addgene inc)


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    Structured Review

    Addgene inc mutant tcf lef reporter plasmid
    Mutant Tcf Lef Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+tcf+lef+reporter+plasmid/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/pm32428834-65-35-43
    Average 94 stars, based on 258 article reviews
    mutant tcf lef reporter plasmid - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Cyclin G2 suppresses Wnt/β-catenin signaling and inhibits gastric cancer cell growth and migration through Dapper1
    Article Snippet: .. The TCF/LEF reporter plasmid (M50 Super 8× TOPFlash, plasmid #12456; Addgene, Cambridge, MA, USA) and the mutant TCF/LEF reporter plasmid (M51 Super 8× FOPFlash, plasmid #12457; Addgene) were gifts from Dr. Randall T. Moon [ ]. .. The Renilla luciferase control plasmid was purchased from Promega (Madison, WI, USA).

    Article Title: Inhibition of Wnt/β-catenin pathway reverses multi-drug resistance and EMT in Oct4 + /Nanog + NSCLC cells.
    Article Snippet: After 7 days of culture, the number of spheres (diameter> 50 μm) was counted under a microscope (Nikon, Japan). .. The cells (0.8 × 105 cells/well) were seeded in 24-well plates and cultured for 24 h. Cells were transiently co-transfected 0.5 μg TCF/LEF reporter plasmid (M50 Super 8× TOPFlash, plasmid #12456; Addgene, USA) or the mutant TCF/LEF reporter plasmid (M51Super 8×FOPFlash, plasmid #12457; Addgene, USA) and 0.05 μg Renilla luciferase plasmid (pRL-SV40 vector, Promega, USA) for normalization, using X-tremeGENE HP DNA Transfection Reagent (Roche, Germany). .. After transfection for 24 h, the cells were lysed in passive lysis buffer and harvested for assays of luciferase activity (Promega, USA).

    Mutagenesis:

    Article Title: Cyclin G2 suppresses Wnt/β-catenin signaling and inhibits gastric cancer cell growth and migration through Dapper1
    Article Snippet: .. The TCF/LEF reporter plasmid (M50 Super 8× TOPFlash, plasmid #12456; Addgene, Cambridge, MA, USA) and the mutant TCF/LEF reporter plasmid (M51 Super 8× FOPFlash, plasmid #12457; Addgene) were gifts from Dr. Randall T. Moon [ ]. .. The Renilla luciferase control plasmid was purchased from Promega (Madison, WI, USA).

    Article Title: Inhibition of Wnt/β-catenin pathway reverses multi-drug resistance and EMT in Oct4 + /Nanog + NSCLC cells.
    Article Snippet: After 7 days of culture, the number of spheres (diameter> 50 μm) was counted under a microscope (Nikon, Japan). .. The cells (0.8 × 105 cells/well) were seeded in 24-well plates and cultured for 24 h. Cells were transiently co-transfected 0.5 μg TCF/LEF reporter plasmid (M50 Super 8× TOPFlash, plasmid #12456; Addgene, USA) or the mutant TCF/LEF reporter plasmid (M51Super 8×FOPFlash, plasmid #12457; Addgene, USA) and 0.05 μg Renilla luciferase plasmid (pRL-SV40 vector, Promega, USA) for normalization, using X-tremeGENE HP DNA Transfection Reagent (Roche, Germany). .. After transfection for 24 h, the cells were lysed in passive lysis buffer and harvested for assays of luciferase activity (Promega, USA).

    Cell Culture:

    Article Title: Inhibition of Wnt/β-catenin pathway reverses multi-drug resistance and EMT in Oct4 + /Nanog + NSCLC cells.
    Article Snippet: After 7 days of culture, the number of spheres (diameter> 50 μm) was counted under a microscope (Nikon, Japan). .. The cells (0.8 × 105 cells/well) were seeded in 24-well plates and cultured for 24 h. Cells were transiently co-transfected 0.5 μg TCF/LEF reporter plasmid (M50 Super 8× TOPFlash, plasmid #12456; Addgene, USA) or the mutant TCF/LEF reporter plasmid (M51Super 8×FOPFlash, plasmid #12457; Addgene, USA) and 0.05 μg Renilla luciferase plasmid (pRL-SV40 vector, Promega, USA) for normalization, using X-tremeGENE HP DNA Transfection Reagent (Roche, Germany). .. After transfection for 24 h, the cells were lysed in passive lysis buffer and harvested for assays of luciferase activity (Promega, USA).

    Luciferase:

    Article Title: Inhibition of Wnt/β-catenin pathway reverses multi-drug resistance and EMT in Oct4 + /Nanog + NSCLC cells.
    Article Snippet: After 7 days of culture, the number of spheres (diameter> 50 μm) was counted under a microscope (Nikon, Japan). .. The cells (0.8 × 105 cells/well) were seeded in 24-well plates and cultured for 24 h. Cells were transiently co-transfected 0.5 μg TCF/LEF reporter plasmid (M50 Super 8× TOPFlash, plasmid #12456; Addgene, USA) or the mutant TCF/LEF reporter plasmid (M51Super 8×FOPFlash, plasmid #12457; Addgene, USA) and 0.05 μg Renilla luciferase plasmid (pRL-SV40 vector, Promega, USA) for normalization, using X-tremeGENE HP DNA Transfection Reagent (Roche, Germany). .. After transfection for 24 h, the cells were lysed in passive lysis buffer and harvested for assays of luciferase activity (Promega, USA).

    Transfection:

    Article Title: Inhibition of Wnt/β-catenin pathway reverses multi-drug resistance and EMT in Oct4 + /Nanog + NSCLC cells.
    Article Snippet: After 7 days of culture, the number of spheres (diameter> 50 μm) was counted under a microscope (Nikon, Japan). .. The cells (0.8 × 105 cells/well) were seeded in 24-well plates and cultured for 24 h. Cells were transiently co-transfected 0.5 μg TCF/LEF reporter plasmid (M50 Super 8× TOPFlash, plasmid #12456; Addgene, USA) or the mutant TCF/LEF reporter plasmid (M51Super 8×FOPFlash, plasmid #12457; Addgene, USA) and 0.05 μg Renilla luciferase plasmid (pRL-SV40 vector, Promega, USA) for normalization, using X-tremeGENE HP DNA Transfection Reagent (Roche, Germany). .. After transfection for 24 h, the cells were lysed in passive lysis buffer and harvested for assays of luciferase activity (Promega, USA).



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